List of non-binary characters in animation

This is a list of non-binary characters in animation that either self-identify as non-binary (i.e. genderqueer) or have been identified by outside parties to be of the non-binary gender, agender, bigender, genderfluid, genderqueer, as well as characters of any third gender. Listed characters are either recurring characters, cameos, guest stars, or one-off characters in animated series, but not animated films. This article also includes characters in Japanese animation, otherwise known as anime.

For fictional characters in other parts of the LGBTQ community, see the lists of gay, trans, lesbian, bisexual, pansexual, asexual, and intersex characters.

The names are organized alphabetically by surname (i.e. last name), or by a single name if the character does not have a surname. If more than two characters are in one entry, the last name of the first character is used. These characters are organized by their identities within the non-binary umbrella of identities, rather than by years, as corresponding lists of lesbian, bisexual and gay animated characters, do.