List of bisexual characters in animation

This is a list of characters in animation that either self-identify as bisexual or have been identified by outside parties to be bisexual. Listed characters are either recurring characters, cameos, guest stars, or one-off characters in animated series, but not animated films. This article also includes characters in Japanese animation, otherwise known as anime. There are also corresponding lists of lesbian, non-binary, and gay animated characters.

For fictional characters in other parts of the LGBTQ community, see the lists of gay, trans, lesbian, non-binary, pansexual, asexual, and intersex characters.

The names are organized alphabetically by surname (i.e. last name), or by a single name if the character does not have a surname. If more than two characters are in one entry, the last name of the first character is used.