2025 US Open – Women's singles qualifying

The 2025 US Open – Women's singles qualifying was a series of tennis matches that took place from 18 to 22 August 2025 (was originally to end on 21 August, but got rescheduled due to heavy rain) to determine the sixteen qualifiers into the main draw of the women's singles tournament.

Sada Nahimana was the first Burundian player to compete in the qualifying competition of a major, and the first black African player from Africa in history to do so.

Janice Tjen was the first Indonesian to compete in the main draw of a singles major, following her qualification, since Angelique Widjaja at the 2004 US Open.

Only 16 out of the 128 qualifiers who competed in this tournament secured a main draw place, and, if necessary, the lucky losers.