2025 Knoxville Challenger – Singles
| Singles | |
|---|---|
| 2025 Knoxville Challenger | |
| Champion | Mitchell Krueger |
| Runner-up | Darwin Blanch |
| Score | 6–7(2–7), 6–4, 6–1 |
Christopher Eubanks was the defending champion but chose not to defend his title.
Mitchell Krueger won the title after defeating Darwin Blanch 6–7(2–7), 6–4, 6–1 in the final.