2025 Knoxville Challenger – Singles

Singles
2025 Knoxville Challenger
Champion Mitchell Krueger
Runner-up Darwin Blanch
Score6–7(2–7), 6–4, 6–1

Christopher Eubanks was the defending champion but chose not to defend his title.

Mitchell Krueger won the title after defeating Darwin Blanch 6–7(2–7), 6–4, 6–1 in the final.