Tetrahydrocolumbamine 2-O-methyltransferase

tetrahydrocolumbamine 2-O-methyltransferase
Identifiers
EC no.2.1.1.89
CAS no.93792-09-7
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO
Search
PMCarticles
PubMedarticles
NCBIproteins

In enzymology, tetrahydrocolumbamine 2-O-methyltransferase (EC 2.1.1.89) is an enzyme that catalyzes the chemical reaction:

(S)-isocorypalmine
+ SAM
 
 
 
 
 
 
 
+ SAH
 

In this methylation reaction, the enzyme uses the cofactor, S-adenosyl methionine (SAM) which transfers a methyl group, giving S-adenosyl-L-homocysteine (SAH) as a by-product. There are several similar transformations in the chemistry of isoquinoline alkaloids.

This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. The systematic name of this enzyme class is S-adenosyl-L-methionine:5,8,13,13a-tetrahydrocolumbamine 2-O-methyltransferase. This enzyme is also called tetrahydrocolumbamine methyltransferase. This enzyme participates in alkaloid biosynthesis.