Q65 (New York City bus)

The Q65 bus route constitutes a public transit line in Queens, New York City. The south-to-north route runs on 164th Street, operating between two major bus-subway hubs: Sutphin Boulevard–Archer Avenue station in Jamaica and Flushing–Main Street station in Flushing. The route is city-operated under the MTA Bus Company brand of MTA Regional Bus Operations.

The bulk of the bus route between Jamaica and Flushing follows a former streetcar line known as the Flushing–Jamaica Line, Jamaica–Flushing Line, or 164th Street Line, operated by the New York and Queens County Railway from 1899 to 1937. Until 2025, there was an extension of the route following a second line operated by the company called the College Point Line or Flushing–College Point Line, which began operation in 1891. Both lines, combined known as the Jamaica–College Point Line or Jamaica−Flushing−College Point Line, were replaced by bus service in 1937, operated by successor companies Queens-Nassau Transit Lines, Queens Transit Corporation, and finally Queens Surface Corporation until the route was taken over by the city in 2005. On June 29, 2025, the route was shortened to Flushing at its northern end, and service on College Point Boulevard was replaced by the Q26.