| Otothyrinae
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| Hisonotus acuen
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| Scientific classification
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| Kingdom:
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Animalia
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| Phylum:
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Chordata
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| Class:
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Actinopterygii
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| Order:
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Siluriformes
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| Family:
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Loricariidae
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| Subfamily:
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Otothyrinae
Chiachio, C. de Oliveira & Montoya-Burgos, 2008
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| Genera
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- Corumbataia Britski, 1997
- Curculionichthys Roxo, Silva, Orrego & Oliveira, 2015
- Epactionotus Reis & Schaefer, 1998
- Eurycheilichthys Reis & Schaefer, 1993
- Hisonotus Eigenmann & Eigenmann, 1889
- Microlepidogaster Eigenmann & Eigenmann, 1889
- Otothyris Myers, 1927
- Otothyropsis Ribeiro, Carvalho & Melo, 2005
- Parotocinclus Eigenmann & Eigenmann, 1889
- Pseudotocinclus Nichols, 1919
- Pseudotothyris Britski & Garavello, 1984
- Schizolecis Britski & Garavello, 1984
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Otothyrinae is a subfamily of South American catfishes of the family Loricariidae. Alternatively it is treated as a tribe in Hypoptopomatinae.