Undecaprenyl-diphosphatase

In enzymology, an undecaprenyl-diphosphatase (EC 3.6.1.27) is an enzyme that catalyzes the chemical reaction

undecaprenyl diphosphate + H₂O ${\displaystyle \rightleftharpoons }$ undecaprenyl phosphate + phosphate

Thus, the two substrates of this enzyme are undecaprenyl diphosphate and H₂O, whereas its two products are undecaprenyl phosphate and phosphate. The enzymatic activity is enhanced by divalent cations, particularly Ca²⁺.

In many bacteria, this enzyme is a membrane protein that participates in peptidoglycan biosynthesis. The enzyme has been implicated in conferring resistance to the antibiotic bacitracin.