Chemiluminescent immunoassay

Chemiluminescence immunoassay (CLIA) is a type of immunoassay that uses chemiluminescence (CL) to determine the concentration of an analyte. This is achieved through the addition of a luminophore or enzyme marker to the antibody, allowing it to form an immune complex with the target antigen. The addition of a luminescent substrate results in a chemical reaction that produces chemiluminescence. The intensity of emitted light corresponds to the concentration of the analyte.

This process is used in clinical diagnostics as well as food and environmental analysis due to its high specificity and ability to detect low concentrations. CLIA is still being improved with research in nanoparticles and ways to improve its efficiency and accuracy.

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