Centromere protein L (CENPL or CENP-L) is a protein that in humans is encoded by the CENPL gene. It is known for its role in forming the centromere and building a functional kinetochore during cell division. CENPL acts as part of the CENP-L/N complex and also contributes to larger centromeric groups such as CENPA-CAD and CENP-H/I/K/L/M subcomplexes, which belong to the constitutive centromere-associated network (CCAN). These proteins work together to recognize CENPA-containing nucleosomes and help establish the site where the kinetochore will assemble, supporting accurate chromosome segregation during mitosis.