2025 US Open – Women's singles

Defending champion Aryna Sabalenka defeated Amanda Anisimova in the final, 6–3, 7–6^(7–3) to win the women's singles tennis title at the 2025 US Open. It was her second US Open singles title and fourth major singles title overall. Sabalenka was the first woman to defend the title since Serena Williams in 2014. She retained the world No. 1 singles ranking after reaching the quarterfinals; Iga Świątek and Coco Gauff were also in contention for the top ranking at the beginning of the tournament.

For the third time at a major in the Open Era, all eight quarterfinalists had previously reached the final of a major, along with the 1976 and 1981 Wimbledon Championships.

Victoria Jiménez Kasintseva became the first Andorran player to compete in a major main draw. Alexandra Eala became the first Filipino player in the Open Era to win a singles main draw match at a major. Renata Zarazúa became the first Mexican woman to ever defeat a Top 10 opponent at the US Open.

Venus Williams was the oldest woman to compete in the US Open singles main draw since Renée Richards in 1981, after receiving a wildcard into the main draw.

This tournament marked the final professional appearances of former world No. 2 and two-time Wimbledon champion Petra Kvitová, who lost in the first round to Diane Parry, and former world No. 4 and WTA Finals champion Caroline Garcia, who lost in the first round to Kamilla Rakhimova.