2025 U.S. Open Cup

The 2025 Lamar Hunt U.S. Open Cup was the 110th edition of the U.S. Open Cup, the knockout domestic cup competition of American soccer, organized by the United States Soccer Federation. It involved 96 teams, including 64 teams from all three professional tiers of the United States soccer league system, and 32 amateur teams in the "Open Division". The competition began on March 18 and culminated in the final on October 1. The defending champions, Los Angeles FC, were represented by their reserve team, and were eliminated in the second round by extension side AV Alta of USL League One.

The full format was announced on January 28, 2025. U.S. Soccer adopted new rules for the competition that restricted the use of alternative venues, and had random draws for hosting beginning in the Round of 16.

Of the 27 American clubs in Major League Soccer, 24 participated, with teams that were playing in the 2025 CONCACAF Champions Cup being represented by their reserve teams. The first teams entered in the round of 32 (the fourth round), while the eight affiliated reserve teams and the two independent teams from MLS Next Pro participated beginning in the first round. Atlanta United FC, Colorado Rapids, and expansion San Diego FC did not have any representation from their clubs in the 2025 U.S. Open Cup. Half of the MLS first team entrants hosted their first matches, while the rest began with an away game. The MLS Next Pro teams were able to field players from their senior MLS team's supplemental roster via loans. The USL Championship sent all 24 of its teams, with the highest sixteen rated teams of the 2024 regular season entering in the Third Round, and the eight lower-seeded teams entering in the first round alongside all 14 teams from USL League One. The 32 Open Division teams entered in the first round.

The winner, Nashville SC, qualified for the 2026 CONCACAF Champions Cup.