2025 Prague Open – Singles
| Singles | |
|---|---|
| 2025 Prague Open | |
| Champion | Marie Bouzková |
| Runner-up | Linda Nosková |
| Score | 2–6, 6–1, 6–3 |
| Draw | 32 (6Q / 4WC) |
| Seeds | 8 |
Marie Bouzková defeated Linda Nosková in the final, 2–6, 6–1, 6–3 to win the singles tennis title at the 2025 Prague Open. It was her second career WTA Tour singles title, and her second title at the tournament, after 2022.
Magda Linette was the reigning champion, but chose to compete in Washington instead.
Eight Czech players reached the round of 16, a record for a WTA Tour event.