2025 National Bank Open – Women's singles

Victoria Mboko defeated Naomi Osaka in the final, 2–6, 6–4, 6–1 to win the women's singles tennis title at the 2025 Canadian Open. It was her first WTA Tour title, and she saved a match point en route, in the semifinals against Elena Rybakina. Mboko was the second wildcard in the Open Era to win the tournament, after Monica Seles in 1995, the third Canadian woman to win the tournament (after Faye Urban in 1969 and Bianca Andreescu in 2019), and the third woman to win a WTA 1000 event as their first tour-level title (after Osaka and Andreescu at the 2018 and 2019 Indian Wells Opens). Ranked as the world No. 85, Mboko was the lowest-ranked champion at the Canadian Open and second-lowest ranked woman to win a WTA 1000 title, after No. 133 Kim Clijsters at the 2005 Indian Wells Open.

Jessica Pegula was the two-time defending champion, but lost in the third round to Anastasija Sevastova.

With her wins over Sofia Kenin, Coco Gauff, Rybakina, and Osaka, Mboko was the second-youngest woman in the Open Era to defeat four major champions at the same tournament, after Serena Williams at the 1999 US Open. For the third time overall in the Open Era (after 1977 and 1979), two unseeded women contested the final. This was also the first time since the current WTA 1000 format was established (since 2009) that two players ranked outside the top 40 contested a WTA 1000 final.

This event marked the last professional appearance of former world No. 5 and 2014 Wimbledon finalist Eugenie Bouchard. She lost in the second round to Belinda Bencic.