2025 Mubadala Citi DC Open – Women's singles
| Women's singles | |
|---|---|
| 2025 Mubadala Citi DC Open | |
| Champion | Leylah Fernandez |
| Runner-up | Anna Kalinskaya |
| Score | 6–1, 6–2 |
| Draw | 28 (4Q / 4WC) |
| Seeds | 8 |
Leylah Fernandez defeated Anna Kalinskaya in the final, 6–1, 6–2 to win the women's singles tennis title at the 2025 Washington Open. It was her fourth career WTA Tour title.
Paula Badosa was the reigning champion, but withdrew before the tournament.
With her win over Peyton Stearns in the first round, 45-year-old Venus Williams became the oldest woman to win a WTA Tour-level singles match since Martina Navratilova at the 2004 Wimbledon Championships.