2024 National Bank Open – Women's singles

Defending champion Jessica Pegula defeated Amanda Anisimova in the final, 6–3, 2–6, 6–1 to win the women's singles tennis title at the 2024 Canadian Open. It was her third WTA 1000 title and sixth career WTA Tour title. Pegula was the first player to defend the title since Martina Hingis in 2000. Ranked No. 132, Anisimova was the lowest-ranked finalist at the event since Alycia Moulton in 1984.

This marked the second WTA 1000 tournament to have five quarterfinalists from the same country (the United States), the second such instance since the WTA 1000 format was first introduced in 2009 (after the 2009 China Open), and the most American quarterfinalists at the same WTA Tour event since 2001.