2000–01 Gamma Ethniki
| Season | 2000–01 |
|---|---|
| Champions | Patraikos |
| Promoted | |
| Relegated | |
2001–02 → | |
The 2000–01 Gamma Ethniki was the 18th season since the official establishment of the third tier of Greek football in 1982 and under the name Second Division (Greek: Β΄ Κατηγορία). Patraikos was crowned champion, thus winning promotion to Beta Ethniki. Chalkidona-Near East also won promotion after a winning a promotion play-off in which was participated with Atromitos and Ethnikos Piraeus.
Acharnaikos, Naoussa, Kozani, Ampelokipi, Lamia, Veria, Anagennisi Karditsa, ILTEX Lykoi, Pierikos and Ialysos Rodos were relegated to Delta Ethniki.