1964 Campeonato Nacional Segunda División
| Season | 1964 |
|---|---|
| Country | Chile |
| Champions | O'Higgins |
| Runner up | Lister Rossel |
| Promoted | O'Higgins |
| Relegated | None |
| Top goalscorer | Mario Desiderio and Dalmacio San Martín (19 goals) |
← 1963 1965 → | |
The 1964 Segunda División de Chile was the 13th season of the Segunda División de Chile.
O'Higgins was the tournament's champion.